Francis, who made his name caring for the poor in Buenos Aires, Argentina, has won plaudits from Catholics and secularists alike for his impassioned plea to ameliorate suffering in the poorest corners of the world.
Before each sacrifice, fish were weighed and euthanized in 300 mg/l concentration of tricaine methanesulfonate (MS-222, Sigma) to ameliorate suffering.
All efforts were made to ameliorate suffering following previously described husbandry protocols (see [12]).
Animal welfare and steps were taken to ameliorate suffering in accordance with the recommendations of the Weatherall report.
Animals were allowed access to food and water ad libitum and were humanely sacrificed as necessary to ameliorate suffering.
Animals were allowed access to feed and water ad libitum under same normal conditions and were humanely sacrificed as necessary to ameliorate suffering.
If the mice decompensated, they were immediately euthanized by CO2 and secondarily with pneumothorax induction with a scalpel to ameliorate suffering.
All occurrences of illness or injury among study subjects were immediately reported to and treated by CNPRC veterinary staff, and all efforts were made to ameliorate suffering.
In addition, steps were taken to ameliorate suffering in accordance with the recommendations of the Weatherall report, "The Use of Non-human Primates in Research".
Because samples were collected entirely non-invasively from free-living primates, steps to ameliorate suffering in accordance with the recommendations of the Weatherall report, "The use of non-human primates in research," were not relevant to this study.
