Williams's latest score is one the most compelling in his forty-year "Star Wars" career: Rian Johnson's film complicates and enriches the familiar template, and Williams responds with intricate, ambiguous variations on his canon of themes.
This is exemplified by the LIG_PCNA motif, which returns 13 ambiguous variations of the defined ELM (data not shown), the third of which (Q.[IL].SFF) covers all defined positions of the ELM.
These cases occur when there are two sets of alleles, denoted A1 and A2, satisfying the following conditions: The union of variations defining alleles from A 1 is identical to the union of variations defining alleles from A 2. Ambiguous variations from those sets are not close enough to be covered by the paired-end reads originating from only one allele.
The union of variations defining alleles from A1 is identical to the union of variations defining alleles from A2. Ambiguous variations from those sets are not close enough to be covered by the paired-end reads originating from only one allele.
The dataset consists of non-ambiguous variations: each mutated residue in a protein chain is univocally associated either to a cancer or other germline disease.
In these cases, no variant can be detected in one of the read types (i.e. the consensus sequence is non-ambiguous in one of the read types), but the opposing read type exhibits variation (ambiguous consensus) at a given position (e.g. T/T/W).
Ambiguous single nucleotide variations (more than one nucleotide variation at a given nucleotide position) were filtered out.
It is an open problem because of ambiguous boundaries, large variation in intensity distribution, variability of liver geometry from patient to patient and presence of noise.
Thus, explanatory notes can always be used, as illustrated in Fig. 12. Fig. 12 Solving ambiguous situations with variations.
Possible explanations for this ambiguous clustering may be variation in lifetime environmental influences (e.g. life style or the conditions under which the patients had died) and genomic-background effects for individual patients, which would contribute to population expression variation (i.e. noise).
