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Yet, in seeming contrast, Chida, Hamer, Wardle, and Steptoe [ 19] investigated the contribution of positive (i.e., fighting spirit and social support), ambiguous (locus of control and extroversion), and negative (stressful life events and negative coping style) stress-related factors to cancer incidence and mortality.
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Phoradendron diguetianum showed four loci (Phca0GH, PhcaCC3, Phca63B, and PhcaW15) with ambiguous locus-specific amplification, and the loci PhcaGQL, PhcaK4A, and PhcaSRK were monomorphic; P. juniperinum showed no PCR amplifications.
These were classed as ambiguous loci (Table 3).
Two methods were applied based on either allele or haplotype frequencies to infer genotypes at ambiguous loci.
For any ambiguous loci that cannot be phased using the available information, MERLIN software provides output for all possible patterns of gene flow.
In order to solve the correct map position for these ambiguous loci we examined the GenCall ratios as discussed above (Table 2).
Ambiguous loci (by definition) could not be confidently characterized as neutral or non-neutral given conflicting test results and were therefore excluded from subsequent evaluations of genetic differentiation.
This indicates that, for the specific design considered here, the probabilities derived from family information used in the Single_Step were more useful to infer genotypes at ambiguous loci than LD information.
The same recommendation is likely to apply to other organisms and other alignment-ambiguous loci.
There were 7 ambiguous single-locus genotypes for queens of V. acadica, 3 for V. vidua, and 2 for V. atropilosa.
In addition to the mutually overlapped transcripts, the sequence reads are usually mapped to multiple nonoverlapping transcripts, resulting in ambiguous mapped loci.
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