Exact(2)
Codon frequencies were set as free parameters (CodonFreq = 3) and ambiguous columns in the alignment were removed from the analysis.
All genomes of the ST239 representative strains were aligned using the progressive Mauve method with default parameters, and core genome SNPs were retrieved from aligned regions excluding gapped ambiguous columns.
Similar(57)
Since conserved domain II is entirely under strong negative selection and contained only one ambiguous column in the alignment (see Additional file 3(A)), it is likely functionally important in all of the sequences analyzed.
This clade was hardly supported (57%) after exclusion of alignment-ambiguous columns, but received moderate to strong support (79 90%) from analysis of the three complete alignments, respectively.
M. intermedium on Scabiosa formed a monophyletic group, unsupported if alignment-ambiguous columns were excluded but supported by bootstrap values ranging from 88% (MAFFT) to 99% (POA), otherwise, together with M. pinguiculae and M. betonicae, parasites in the flowers of Lentibulariaceae and Lamiaceae, respectively.
Gaps and columns with ambiguous characters were removed.
Columns containing ambiguous sites were removed from the alignment and gaps were treated as fifth state.
Geneious was then used to create a consensus sequence for each individual, to align those consensus sequences, and to remove alignment columns containing ambiguous sites and gaps, respectively.
The New Yorker, June 24 , 1950P. 85 The writer quotes an ambiguous passage from Leonard Lyons' column and asks a number of questions about it.
Conceding that the term "fifth column" was "ambiguous," Saint-Just went on to say that, "the National Front has always said it would combat radical Islam and sectarianism, but never Islam".
Two-column layouts can create ambiguous reading orders; users can go across horizontally or down vertically, and avoid using two columns.
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