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In each assignment attempt we allowed two possible outcomes: (i) A "positive" assignment (i.e. the query was assigned to a taxon) or (ii) An "ambiguous" assignment (i.e. the query was not assigned to a taxon).
With an assumed genotyping error rate of 1%, 99% of the offspring (i.e. 123 individuals) were successfully assigned to a single parental pair with the single ambiguous assignment remaining.
However, increasing database size also makes it potentially more difficult to assign taxonomy at genus and species levels as the likelihood of ambiguous assignment increases due to larger number of competing sequences in the search space.
This ambiguous assignment takes the foreground in a tale ranging over Tassie's home life and love life — the nest she's just departed and the nest she's hoping to flutter into.
The number of incidences of ambiguous assignment and superfluous signals for good, average, poor and bad data are < 5, 5-10, 11-20 and > 20, respectively.
The number of incidences of ambiguous assignment and superfluous signals for good, average, poor and bad data are < 5, 5-10, 11-20 and > 20 ppm, respectively.
Similar(40)
This included library searches using chemical shifts and handling ambiguous assignments for COSY and HMBC correlations.
Therefore, ambiguous assignments can be caused by the overlapping chemical shifts of numerous different structural moieties [33].
The datasets were judged based on the number of incidences of ambiguous assignments across all the NMR experiments.
These low values reflect the fact that a large number of sequences were included from the Sapotaceae and Laureae, and these yielded ambiguous assignments.
Additionally, we demonstrated that the number of ambiguous assignments could be reduced by the introduction of a third dimension, that is, by using heteronuclear 3D NMR spectra (Fig. 3).
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