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We've had stunning mixes from the LuckyMe crew, BBC AZN Network's Manara, rave revivalist Lone, and the mysterious DJ Sonikku, slamming techno courtesy of Peder Mannerfelt, a brutal beyond belief remix by Gramrcy, and some truly sumptuous ambient bubble-bath from Biosphere.
The heated water bath was (passively) cooled to ambient temperature.
The resulting solution was stirred at 81 °C for 10 min, cooled to ambient temperature, and placed on an ice-bath for 2.5 h.
After addition, the cooling bath was removed and the mixture was allowed to warm to ambient temperature.
After the addition, the ice/water bath was removed and the stirred mixture was allowed to warm to ambient temperature.
Air dry to ambient humidity.
For some selectors this involves taking things down a notch and cranking out a nice bit of warming ambient analogue bubble bath.
Each leaf sample was carefully rolled to fit to the bottom of a 2-mL microcentrifuge tube and then completely submerged with 1.00 mL of an internal standard solution (0.1 mg mL−1 m-xylene in pure ethanol), sonicated in a ambient temperature water bath for 1 min and then briefly vortex mixed.
The prepared fibers were wetted and kept in a tap water bath at ambient temperature for 24 h to eliminate the residual DMAc solvent.
Lipid films were then resuspended with phosphate buffer saline [pH 7.2] to concentration of 4 mM and freeze-thawed 10-times using liquid nitrogen and a water bath at ambient temperature.
A high purity amorphous spinel film (CdCr2S4) electrode composed of monodispersed grains has been successfully deposited on an ordinary glass and conducting glass (FTO) substrates from aqueous medium using a simple, inexpensive, reproducible chemical bath method at ambient temperature.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com