Sentence examples for always harvested from inspiring English sources

Cultures were always harvested in the exponential growth phase, i.e., at densities lower than 2×106 cells/ml for BSF and 2×107 cells/ml for PCF, by centrifugation at 1,000×g for 10 min. Transfection of trypanosomes and selection of clones were performed as described previously [21].

Root materials were always harvested after a total cultivation period of 4 weeks and were dried at room temperature.

Cover slips were always harvested when cells were sub-confluent, fixed with 4% paraformaldehyde in PBS for 20 min at room temperature, and permeabilized with 0.2% Triton X-100 as described [ 11].

MSC were always harvested by trypsinisation upon 80% confluent growth, counted with a Neubauer counting chamber (Brand, Wertheim, Germany) and additionally with a CASY cell counter (Schärfe System, Reutlingen, Germany) and re-seeded in a density of 10,000 cells/cm in culture flasks (Nunc Thermo Fisher Scientific, Langenselbold, Germany).

He was always harvesting information, wanting to know more.

Now, this is not the way we ALWAYS harvest honey.

Lark grows a superior variety, Minar, and most important, always harvests when the cucumbers are small and slender.

They also staggered their operations so they were always harvesting: if one house or flat was raided, the profits from the others more than covered it.

He never ages, the seasons never change, and the crops always need harvesting.

The extraction and analysis of cellular fatty acids were carried out in two independent repetitions from biomass grown on GYM agar plates held at 28°C for 4 days and harvested always from the same sector (the last quadrant streak).

Kansal et al. [40] for instance, assume that the amount of energy being harvested is always known, a reasonable requirement given that the Heliomote [46], for which the algorithm in [40] was designed, is equipped with dedicated circuitry that measures the voltage and current (hence, power and energy) coming from the solar panels.