He was always harvesting information, wanting to know more.
Lark grows a superior variety, Minar, and most important, always harvests when the cucumbers are small and slender.
They also staggered their operations so they were always harvesting: if one house or flat was raided, the profits from the others more than covered it.
Cultures were always harvested in the exponential growth phase, i.e., at densities lower than 2×106 cells/ml for BSF and 2×107 cells/ml for PCF, by centrifugation at 1,000×g for 10 min. Transfection of trypanosomes and selection of clones were performed as described previously [21].
Root materials were always harvested after a total cultivation period of 4 weeks and were dried at room temperature.
Cover slips were always harvested when cells were sub-confluent, fixed with 4% paraformaldehyde in PBS for 20 min at room temperature, and permeabilized with 0.2% Triton X-100 as described [ 11].
MSC were always harvested by trypsinisation upon 80% confluent growth, counted with a Neubauer counting chamber (Brand, Wertheim, Germany) and additionally with a CASY cell counter (Schärfe System, Reutlingen, Germany) and re-seeded in a density of 10,000 cells/cm in culture flasks (Nunc Thermo Fisher Scientific, Langenselbold, Germany).
If you are fiscally irresponsible, always be harvest savvy - and this year's crop of Florida sweet corn is pure gold - literally and figuratively.
We want to do what we have always done, harvest from nature as long as it's sustainable.
