Estrogen receptor alpha (ERα) and progesterone receptor (PR) expression was largely limited to hormone-sensing cells, whereas both YFPpos and YFPneg alveolar progenitor populations exclusively expressed the alveolar marker genes Elf5 and β-Casein[ 26].
In contrast, clonogenic BCs do not express other bronchial or alveolar lineage markers as shown by RNA-Seq and confirmed by immunostaining (Figs. 1F and S1B).
Figure 3E: the authors claimed that their lung organoids contains bipotent alveolar progenitor markers including NMYC, ID2, NKX2.1, SPC, and HOPX based on their q-PCR analysis.
Bronchioalveolar stem cells (BASCs) are another population of naphthalene-resistant stem cells located at the BADJ and can be identified based on their coexpression of secretory (Scgb1a1) and alveolar (Sftpc) markers, [ 19].
We have now conducted this analysis and include the data in Figure 3 figure supplement 3. Figure 3E : the authors claimed that their lung organoids contains bipotent alveolar progenitor markers including NMYC, ID2, NKX2.1, SPC, and HOPX based on their q-PCR analysis.
Dual immunofluorescent staining of viral nucleoprotein and type II alveolar cell marker SP-C protein suggested that the majority of infected alveolar epithelial cells were type II pneumocytes.
To determine the type of virus infected cells in mouse lung alveoli, viral NP protein and type II alveolar cell marker SP-C protein were examined for their co-localization within the infected cells.
In this mouse model, however, reporter studies have shown that β-catenin signaling occurs within a distinct subset of luminal cells scattered along ducts with alveolar progenitor marker profiles and functional characteristics [ 67].
Kondo, H., Miyoshi, K., Sakiyama, S., Tangoku, A. & Noma, T. Differential Regulation of Gene Expression of Alveolar Epithelial Cell Markers in Human Lung Adenocarcinoma-Derived A549 Clones.
Given that SP-C and SP-B are alveolar epithelial cell marker proteins, these results suggest that these elevated serum EVs originate from epithelial cells.
