Reply to Wijmans et al.: Optical Coherence Tomography: A Valuable Novel Tool for Assessing the Alveolar Compartment in Interstitial Lung Disease?
Further BALF examination demonstrated that both ventilation strategies induced cell influx into the alveolar compartment, with higher levels in HVT-ventilated mice.
We also determined the levels of these inflammatory cytokines within BALF and lung homogenates to assess the inflammatory response in the alveolar compartment.
DAMPs are present in the alveolar compartment during (injurious) MV indicating the potential significance of the DAMP/receptor axis in VILI [7].
In our 1-hit VILI model, RAGE deficiency was associated with a reduced total cell and neutrophil influx into the alveolar compartment.
Lung weights, collagen and total protein in BALF, as surrogate endpoints of extravasated capillary fluid into the alveolar compartment accessible by BAL fluid, followed a similar trend (Fig. 3).
To try to better assess PaCO2, we also attempted to sample gas closer to the alveolar compartment by measuring expiratory CO2 at the end of a 'complete' expiration (either active or passive) [14] but this approach was also disappointing.
Each i th alveolar compartment has a unique and configurable bronchiolar resistance R B,i [cmH2O∙s/l], pulmonary vascular resistance R V,i [dyn∙s/cm5], stiffness index S i [cmH2O/ml2], and extrinsic pressure Pext,i [cmH2O](giving 4×N adjustable parameters in total, where N is the number of compartments).
However, the dissolved magnetization is continuously replenished by HP 129Xe diffusing in from the alveolar compartment.
We compared the levels of neutrophil recruitment in the alveolar compartment of ADAM17-null and control mice.
Interestingly, however, neutrophil recruitment to the alveolar compartment occurred earlier in ADAM17-null mice after exposure to LPS.
