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Here, we studied the UV-B regulation of P1 in maize leaves of high altitude landraces, and we investigated how UV-B regulates P1binding to the CHS promoter in both low and high altitude lines.
Thus, it is clear that P1 participates in the UV-B induction of the flavonoid pathway in leaves of high altitude landraces, but not in leaves of low altitude lines.
Although polymorphisms exist between the P1 basal promoter sequences of the landraces and low altitude lines, we found that differences in gene regulation by UV-B are largely a consequence of effects in trans-, suggesthat thesethese regulatory sequence differences are not the causal reasons for the difference in expression.
Because we found that high altitude landraces show P1 induction by UV-B, and higher expression in leaves compared to the low altitude lines B73 and W23, we then cloned and compared P1 promoter sequences from the different landraces to analyze the presence of putative regulatory sequences that may be responsible for the differential patterns of expression.
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P1 expression level was measured in young leaves of five different maize high-altitude landraces and in one low altitude line, W23.
Thus, we analyzed the association of P1 to the promoter region of C2 in one high-altitude landrace, Confite Puneño (CFT), and in the W23 low altitude line under control conditions (without UV-B) and after an 8 h UV-B treatment.
In recent years, Chinese airlines have opened routes into Nepal as the number of Chinese tourists has risen steadily, and Nepalese officials also want China to extend rail services to the border so that Nepal can be linked to the same high-altitude line that connects Beijing to Tibet.
The maps don't account for variations due to altitude, tree lines and ground-level brush.
However, in some high altitude maize lines C-glycosyl flavones can also accumulate in leaves [ 6] indicating genetic diversity for developmental accumulation of flavonoid metabolites.
Fig. 22 Close-up of Fig. 10 around the time period of flight UAV 4 showing its altitude (red line) and the altitude of the Vaisala sonde (blue line) released on June 7 , 2015
The particle flux (panel (a)) and energy flux (panel (b)) are summarized at the exobase in the Parker spiral case at 207 km altitude (solid line with crosses), the inner sphere at 240 km altitude (solid line) and outer sphere at 560 km altitude (dashed line) in the B x = 0 case.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com