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During evolution, new functions are acquired as a consequence of an accumulation of substitutions that alters the coding or regulatory sequences of genes.
Of the unique SNVs and indels in F0, the three SNVs are predicted to be silent mutations, whereas the indel alters the coding sequence of DNA polymerase γ (CNAG_06769).
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Generally, the copy number alteration at gene promoters typically does not alter the coding sequences of genes but contributes to cancer by influencing gene expression [ 21].
These codon modifications altered the coding sequence in the gag reading frame without affecting the protein sequence enabling the expression of Gag but not Gag-Pol.
SNP reported here has been designated rs3730477 (http://www.ncbi.nlm.nih.gov/SNP/snp_ref.cgi rs=3730477), and is one of the two SNPs altering the coding sequence identified to date in hPolλ.
This may alter the coding sequence.
These mutations either alter the coding sequence of tau or the alternative splicing of MAPT.
Most alternative splicing variations are localized to the protein coding sequence and are predicted to significantly alter the coding sequence.
Sequence analysis of ACTC1 and ACTA2 did not identify any nucleotide changes that altered the coding sense of the genes.
Therefore, missense mutations altering the coding sequence in this region are predicted to severely affect protein function.
SSRs with a monomer length that is not multiple of three and located within gene coding regions can significantly alter the coding potential of a given transcript.
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