Exact(1)
The beads were washed 2 3 times with extraction buffer and alternatively twice with wash buffer (50 mM TrisCl, 250 mM NaCl, 0.1% NP 40, 0.05% sodium deoxycholate, pH 7.5).
Similar(59)
All models were tested twice alternatively using D.near and D.centre as isolation measures.
Alternatively, mice were injected twice at 2-week intervals in the footpad with a volume of 50 µl.
Alternatively, it can be given twice a day IV with a loading dose of 6 mg/kg Q12 hours for 1 day, followed by 4 mg/kg BID.
The plants were sub-irrigated twice a week, alternatively with tap water and the appropriate nutrient solution.
This single injection or, alternatively, 4 injections in total (twice a week for 2 weeks) were given.
Alternatively the employer may pay about twice the amount falling to the employee.
Alternatively, peptide-gating might have evolved twice independently in the DEG/ENaC gene family.
Alternatively, the cells were harvested, washed twice with buffer and stained for Annexin V, PI, CD95, CD25, CD71, and CD3 as described above.
(LMGTFY link) Alternatively you could type "z or r twice", which is a reference to the maneuver in Star Fox.
Alternatively, using the dosage of 160/800 mg twice daily for three months has been shown to be effective in eradication of the infection [70].
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