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Alternatively, sections were treated for haematoxylin and eosin staining before capturing images with a Leica DC 500 digital camera mounted on the microscope.
Alternatively, sections were submitted to the TUNEL assay at various time points to determine DNA fragmentation according to manufacturer instructions (In Situ Cell Death Detection kit, POD, Roche Diagnostics, Mannheim, Germany).
Alternatively, sections were labeled with antibodies against cCasp3.
Alternatively, sections were stained using Gomori's trichrome (Sigma) following the manufacturer's recommendations.
Alternatively, sections were stained for von Willebrand factor (vWf) expression to determine vascularity.
Alternatively, sections incubated with the anti-human CD109 antibody as above were detected with a horseradish peroxidase-conjugated rabbit anti-mouse secondary antibody followed by diaminobenzidine staining.
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Alternatively, the sections were antigen-retrieved and blocked with 2.5%% normal horse serum (Vector Labs, Burlingame, CA) and subsequently probed with a mouse monoclonal anti-α-smooth muscle actin (α-SMA) antibody (1 100) (Abcam, Cambridge, MA, USA) overnight at 4 °C, followed by incubation in the ImmPRESS® anti-mouse IgG (Vector).
Alternatively, thin sections of 1 µm were stained by a mixture of toluidine blue and azur II and observed under a Leica DME photonic microscope.
Alternatively, colonic sections were fixed in Accustain (Sigma) at 4° for 4 h and embedded in paraffin.
Alternatively, a section of the TTT curriculum could focus on how core trainers contact and arrange for Grand Rounds lectures.
Immunocytochemistry was in part performed on the entire hemispheric section or, alternatively, in dissected hemispheric sections, containing the lesions, the normal appearing white matter and the cerebral cortex.
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