To alternatively quantify the cellular oxidative stress, the dichlorofluorescein assay (Wang and Joseph, 1999), which measures the oxidation of non-fluorescent dichlorofluorescin derivates into fluorescent dichlorofluorescein derivates by reactive oxygen species, was applied.
Alternatively, to quantify invasion in the mini-organotypic model in which PS1 cells were treated with FGFR1 RNAi, total cell number invading per gel was plotted.
Alternatively, to quantify filopodia sprouts per vessel length NIH ImageJ software was used to count projects per 100 μm at the leading edge of the migrating vessel front (n=3 images per retina, n=3 mice per genotype).
Seven alternatively spliced isoforms were quantified using splice-targeted primers.
Individual ANDS-labeled glycans were quantified, or alternatively scans (tracings) of all glycans in gel lanes were generated electronically, with the Quantity-One software supplied with the imager.
Alternatively, apoptosis was also quantified by flow cytometry after double staining using FITC-conjugated annexin V/propidium iodide (PI) apoptosis detection kit (Eppendorf, Milan, Italy), which allows discrimination between early apoptotic, late apoptotic and necrotic cell.
Alternatively, HCV RNA was quantified on the basis of a real-time reverse transcription polymerase chain reaction using the primer RTU1 [71] for cDNA synthesis, primer pair UTR2 and RTU2 [71] for PCR amplification, and HCV JFH-1 RNA prepared in vitro by means of T7 polymerase as a standard.
Alternatively, fluorescence intensities were quantified in an Odyssey CLx apparatus (Licor).
We have developed the TaqMan®-based AT-qPCR (Alternative Transcript-qPCR) assay to more accurately quantify alternatively spliced mRNA, and demonstrate the measurement of Col2a1 splice form expression in differentiating ATDC5 cells in vitro, and in wild type mouse embryonic and postnatal cartilage in vivo.
Alternatively, one may quantify the 'compatibility', rather than 'similarity' (as measured by the RF distance), among gene trees.
