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Screening our gRNA database, we identified a corresponding gRNA gene that would be able to guide for the insertion of the 3' most alternatively inserted uridine residues at nucleotide position 274-76 (Figure 2B) [15].
In particular, the thermo-anemometric technique and the multi-scale methodology of modelling were applied to investigate air turbulent flow within a rectangular container filled with spheres in cubic arrangement and different baffles alternatively inserted between the spheres.
In addition to this fully edited ND7 sequence, we identified two alternatively edited ND7 mRNAs containing a total of seven alternatively inserted uridines at five editing sites (Figure 1A).
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Alternatively, insert packaging could be impeded by the presence of structural anomalies, such as branched replication or recombination intermediates, within a localized region of the cpDNA.
Alternatively, insert acid-free plastic sheets to hold the recipes in.
Alternatively, the inserted DNA might separate important transcription factor binding sites.
Alternatively, Bax, inserted as monomer in the OMM, destabilised the lipid bilayer, allowing the release of cytochrome c.
In the remaining 11 cases, the predicted TSIP may have been a false prediction or, alternatively, the inserted sequence may have contained difficult to amplify sequences.
The ND8-F04 cDNandnd two other identical sequences contained two regions where a total of 11 uridines are inserted alternatively at seven editing sites (Figure 2A, Table 1).
The variable exons 6 to 5 (known as v1 to 10) can be alternatively spliced and inserted to the standard form between exons 5 and 16 [ 28].
Alternatively, screws could be inserted in carefully drilled holes.
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