Screening our gRNA database, we identified a corresponding gRNA gene that would be able to guide for the insertion of the 3' most alternatively inserted uridine residues at nucleotide position 274-76 (Figure 2B) [15].
In particular, the thermo-anemometric technique and the multi-scale methodology of modelling were applied to investigate air turbulent flow within a rectangular container filled with spheres in cubic arrangement and different baffles alternatively inserted between the spheres.
In addition to this fully edited ND7 sequence, we identified two alternatively edited ND7 mRNAs containing a total of seven alternatively inserted uridines at five editing sites (Figure 1A).
Alternatively, the inserted DNA might separate important transcription factor binding sites.
Alternatively, Bax, inserted as monomer in the OMM, destabilised the lipid bilayer, allowing the release of cytochrome c.
In the remaining 11 cases, the predicted TSIP may have been a false prediction or, alternatively, the inserted sequence may have contained difficult to amplify sequences.
Alternatively, you can insert your whole body into the puppet (Big Bird, the horses in "War Horse") or suspend the puppet from the flies, as with the enormous and frightening figure by Julian Crouch which appears during the battle in Philip Glass's opera "Satyagraha".
Alternatively, each aforementioned insert was subjected to 3′ end-adenine addition through Taq polymerase (Bioline) for ligation at pGEM-T Easy vector multiple cloning site (Promega, WI, USA).
Alternatively you can insert a rod that is slightly shorter than the width of the tapestry.
