Alternatively, assessing localized treatment effects in the individual tumor areas may be possible by using volumetric voxel-by-voxel tumor analysis techniques.
Sperm competition has alternatively been assessed indirectly, by measuring the time interval between successive copulation events [8].
Alternatively, we assessed uncertainty in phylogenetic tree estimation and model parameters with a Bayesian implementation of the GMYC model, which integrates over these potential sources of error via MCMC simulation [ 60].
Alternatively, to assess ADP-ribosylation of eEF-2 in extracts of cells previously treated with toxin, the eEF-2 that was not already modified was quantitated by reaction with biotinylated NAD [9].
3 Alternatively, to assess parsimoniously how the relation changed over years of experience, we specified experience as a set of four linear splines with distinct slopes for each decade of experience and then calculated average marginal effects of the impact of a one year increase in experience on outcomes in their native scale.
Alternatively, viability was assessed with the CellTiter-Glo® Luminescent Cell Viability Assay (Promega Corporation, Madison, WI 53711) at the end of 3 days treatment, and the IC50 values were calculated by GraphPad Software, Inc., La Jolla, CA. Trichostatin A (Sigma) was prepared as 3 mM stock solution diluted in ethanol.
We now included the coverage of alternatively spliced segments assessed in this study in the Discussion.
Alternatively, growth was assessed by MTT using the manufacturers recomendations (Sima-Aldrich).
Alternatively, viability was assessed using the CellTiter-Glo® Luminescent Cell Viability Assay kit (Promega) following the manufacturer's protocol.
Alternatively, ROS generation was assessed in astrocytes cultured on coverslips with the fluorogenic marker carboxy-H 2DCFDA (Molecular Probes, USA) during 30 min/37ºC, protected from the light.
