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Another alternative to alignment to reference libraries is k-mer analysis.
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Recent work has shown that the topology of these graphs captures enough information to allow the detection and characterization of genetic variants, offering an alternative to alignment-based methods.
Kinematic alignment was recently introduced as an alternative to conventional alignment techniques.
The choice for this metric is based on its simplicity, well defined mathematical properties and its demonstrated effectiveness as an alternative to the alignment method (Vinga and Almeida, 2003).
Alignment-free similarity measures offer an alternative to the alignment-based ones in that they are unaffected by such genetic processes.
Connectivity-based strategies could emerge as an alternative to deformation-based alignment leading to match the connectomes of the subjects rather than images.
As an alternative to the multisequence alignment, the SHV variants are visualized as mutations relative to the sequence of SHV-1.
As an alternative to the multisequence alignment, the TEM variants are visualized as mutations relative to the sequence of TEM-1.
Recently, we proposed an alternative approach to alignment-free sequence comparison.
With the complete genome sequences at hand, it appears natural to seek for alternatives to traditional, alignment-based tree-building in the form of integral characteristics of the evolutionary process.
An alternative to using the primary alignment would be to only use uniquely mapped reads, but this would bias the expression values towards older HERV families (see Fig. 7).
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