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An alternative restriction to answer the complaint of "unlimited" German liability would be to require Mr Gauck to attach a proviso limiting Germany's total exposure to the €211bn approved by the Bundestag.
Because three restriction groups were not homogenous, we used alternative restriction endonucleases to distinguish the different subgroups.
Similarly, the PB2 gene was amplified using PB2-For: 5'-CTAGG ACGTC GATGG AACGC ATTAA AGAAC TGCGC AACCT G-3' and PB2-Synth-Rev3: 5'- CCCGT TCATT ATTCG TGCCA TTCGA TTTTC TGAGC CTCGA AGATG TCGTT CAGCC CandG-3' and cloned into alternative restriction sites of pYUM6002 to give plasmid pYAN009 for C-terminal truncation (3' DNA deletion) library construction (Fig. S1A).
Selection of alternative restriction enzymes may therefore also be used to fine tune the performance of WGP in wheat.
Alternative restriction enzymes would be necessary to identify all PTKs according to their unique specific restriction fragment.
In many cases, several different kinases could be represented by similar size restriction fragments, and alternative restriction enzymes would be necessary to identify single-gene-specific restriction fragments.
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It also found the full curfew would cost airlines, passengers, cargo carriers and general aviation $48 million, compared to $18 million and $41 million respectively for the alternative restrictions.
Hoynes (1996) and later work estimates multinomial logit models and relaxes the Independence of Irrelevant Alternatives restriction by including an additive stochastic term in the utility associated with each possible choice.
Furthermore, such a system can be exploited to genotype the heterozygous and homozygous mutant progenies as an alternative RFLP (restriction fragment length polymorphism) without requiring specific restriction enzymes and recognized sites (J. M. Kim et al. 2014).
We also checked the robustness of our results against alternative sampling restrictions and model specifications.
However, multinomial logit models impose the inconvenient "independence of irrelevant alternatives" (IIA) restriction.
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