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We find that RNA-Seq allelic ratios from standard alignment methods correlate poorly with SNaPshot, but applying alternative alignment strategies and correcting for observed biases significantly improves correlations.
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We also explored an alternative, spliced alignment strategy for finding TICs by identifying splicing events within individual reads using our GSNAP program [ 27].
Both alignment strategies yielded similar total mapped reads and coverage.
Similar to sequence alignment strategies, graph alignment can be local or global.
Such a targeted alignment strategy has been highly effective in studying the extent of intragenic alternative splicing in the human genome, even in short reads of only 32 nt [ 20- 23].
Minami, S., Sawada, K. & Chikenji, G. MICAN: a protein structure alignment algorithm that can handle multiple-chains, inverse alignments, C only models, alternative alignments, and non-sequential alignments.
This alignment was used as a reference to which the alternative alignments are compared.
The alignment strategy implemented was L-INS-i [36].
However, redundancy does not necessarily imply alternative alignments.
Thus, only different maximum locations are indicative of alternative alignments.
Our alignment strategy is outlined in Figure 22.
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