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Non lethal Plasmodium yoelii 17XNL (clone 1.1) [4] was maintained by alternating passage of the parasites in A. stephensi mosquitoes and CD-1 mice (Harlan Laboratories, IN).
The capacitance probe registers the alternating passage of bubbles and dense phase at the probe, and the respective contributions to the local instantaneous heat transfer from the gas and particle convection were thus identified by comparison with the heat transfer signal.
In correlation with the measured neutralizing antibody escape in the original studies, we noticed a significantly higher total variance in the p7 HeLa mammalian cell passaged E2 protein (1.6603) when compared with the mammalian-insect alternating passage (1.4262) (one tailed t-test, P = 0.00004) and the starting stock virus (1.3587) (one tailed t-test, P = 0.00018).
In analyzing this batch of samples, we assessed all the positions across the viral genome in which there was a significant variant in the alternating passage sample that had cycled through mosquito cells, which was not found in any of the other samples (that encountered only mammalian cells) above a rate of 0.001.
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Philippe himself, dressed in military uniform and a purple sash, delivered a well-crafted speech with alternating passages in Dutch, French and German.
This was the standard form of inscriptions on stone; its employment in hagiographic literature may have influenced the later development of the chantefable in alternating passages of prose and verse.
In alternating passages, he describes the wonders Cook found on various virgin shores, then reports on the state of each place today.
The figures are lit by a soft, flat, frontal light which allows them to be rendered in terms of alternating passages of cool and warm colors, clustering around a middle gray.
To simulate successive infection cycles, culture supernatant recovered 7 days after transfection of the C6/36 cells was passaged at intervals of 7 days in C6/36 mosquito cells, HuH7 human liver cells or in alternating passages of C6/36 and HuH7 cells.
We Applied ViVan on three sequenced samples: the initial virus population (stock virus); following seven passages in HeLa cells (p7 HeLa); and after alternating passages between HeLa cells and mosquito C6/36 cells (p7 alt).
Alternate passage of extracellular virus in human and mosquito cells did not eliminate particles containing sub-genomic RNA.
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