The phrase "alternating laser" is correct and usable in written English.
It can be used in contexts related to technology, physics, or engineering, particularly when discussing lasers that switch between different states or modes.
Example: "The research team developed an alternating laser system to improve the efficiency of the optical communication network."
Alternatives: "pulsating laser" or "switching laser".
Finally, alternating laser excitation (ALEX) can perform "photophysical sorting" to discern dynamics from photophysics when two-color systems are being used.
Dynamic co-localisation experiments using dual view technology and msALEX microscopy (millisecond alternating laser excitation, Baumann et al., 2014) revealed that Upa1-Gfp co-localises extensively with motile Rab5a-positive endosomes.
For example, Weiss and co-workers have used single-molecule FRET to observe dynamics of single proteins on millisecond time scales, using an alternating laser excitation (ALEX) instrumental scheme that allows singly labeled (donor-only and acceptor-only) molecules to be distinguished from the two double-labeled states (17).
In order to treat hemangioma and to make the reconstruction of cloaca possible, corticosteroid treatment orally and multiple laser treatments were performed alternating Nd YAG laser and pulsed dye laser therapy.
First, a resting-state session of 10 min without any laser stimulation; second, a "block" sequence of 12 min with alternating "laser-on" and "laser-off" periods lasting 30 s each; third, a session of 10 min with continuous laser stimulation; fourth, another resting-state session of 10 min without any laser stimulation.
In addition to Cy3 fluorescence (green) and sensitized emission of Cy5 (FRET, blue) under 532 nm laser excitation, Cy5 fluorescence (red) was collected under alternating 640 nm laser excitation.
To elucidate the relationship between FcγRI binding affinity and the structural dynamics of the upper CH2 region of Fc induced by the CH3 mutations, the conformational variation of Fc variants was observed by single-molecule Förster resonance energy transfer (FRET) analysis using alternating-laser excitation (ALEX).
Instead of PIE, an ALternating-Laser Excitation (ALEX) scheme could have been implemented to obtain a two-laser excitation of the sample [35].
Single-molecule measurements were performed at room temperature using a confocal microscope with alternating-laser excitation between a 532-nm and a 638-nm laser, as described (Doose et al. 2007; Selvin and Ha 2008).
Two-color STED time series were also obtained sequentially line by line by alternating the excitation lasers and the STED laser tuned to 760 nm for both ATTO647N and Chromeo494.
By alternating the excitation laser line by line, the two channels can be recorded quasi-simultaneously, minimizing temporal shift between the images.As a biological application, we fused EGFR with the SNAP-tag and its ligand, EGF, with the CLIP-tag (both New England Biolabs, Ipswitch, MA).
