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Alternate passage of extracellular virus in human and mosquito cells did not eliminate particles containing sub-genomic RNA.
The multistep doxorubicin-selected MCF-7 cells were cultured in high-dose doxorubicin (860 n M) every alternate passage as previously described (Mehta, 1994).
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Paramount has been shooting alternate passages here and there in a movie to be called "Arise My Love".
By T. Robinson and Wolcott Gibbs The New Yorker, August 24, 1940 P. 11 Paramount has been shooting alternate passages here and there in a movie to be called "Arise My Love".
Drawing on his mother's wartime diaries, the Scottish poet and novelist Andrew Greig has produced a poignant evocation of wartime Britain as seen through the eyes of Stella and Len, who narrate alternate passages.
The resulting MCF-7 cell line with paclitaxel resistance, designated MCF-7TaxR, was maintained at 6 nM paclitaxel with treatment at alternate passages.
The senescence of primary MEFs was assayed at alternate passages up to passage 10, using the Senescence β-Galactosidase Staining Kit (Cell Signalling), which exploits pH-dependent β-Galactosidase activity as a readout for senescence.
BSR-T7/5 cells (a BHK derivative that stably expresses T7 RNA polymerase) [ 27], were maintained in Glasgow Minimal Essential Medium (GMEM) supplemented with 4% tryptose phosphate broth, 10% FBS, and in alternate passages geneticin was added to 1 mg/ml to ensure maintenance of the T7 polymerase gene.
The capacitance probe registers the alternating passage of bubbles and dense phase at the probe, and the respective contributions to the local instantaneous heat transfer from the gas and particle convection were thus identified by comparison with the heat transfer signal.
Non lethal Plasmodium yoelii 17XNL (clone 1.1) [4] was maintained by alternating passage of the parasites in A. stephensi mosquitoes and CD-1 mice (Harlan Laboratories, IN).
In correlation with the measured neutralizing antibody escape in the original studies, we noticed a significantly higher total variance in the p7 HeLa mammalian cell passaged E2 protein (1.6603) when compared with the mammalian-insect alternating passage (1.4262) (one tailed t-test, P = 0.00004) and the starting stock virus (1.3587) (one tailed t-test, P = 0.00018).
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