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Replacing the antibody with a low-cost alternate affinity reagent would reduce the costs of diagnostic development and use, and lead to new portable diagnostic devices towards many diseases.
DcAvd provides a scaffold that can be modified not only to alternate affinity for a ligand, but also to change specificity – in other words to favour another ligand molecule instead of biotin.
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These two examples show that alternating successive affinity selections of phages and growth of phage-infected cells in the presence of antibiotic permits the selection of BHPs exhibiting both a high affinity for the target antigen and an efficient enzymatic activity.
Both alternate strategies for affinity increase, one based on faster binding, the other on a slower release of the complexed molecules, have been reported.
Despite these similarities to T cells, no study has evaluated if the decrease in erythrocytes might be a direct or indirect consequence of an alternated co-receptor affinity [ 16].
The alternate behaviours in electron affinity, bond length, and incremental binding energy with odd and even "n" match the peak pattern observed in the laser-induced mass spectra of CnP5− (n=1–7) and the results of experimental reactions.
A recent study shows that the receptor tyrosine kinase EphA4 and its ligand EfnB2a independently mediate this cell affinity in alternating segments of the hindbrain [ 26].
However, we did not observe any clear relationship between alternate exon usage and actin affinity for isoforms.
In particular, it was studied the possibility to realize alternated structures stabilized by binding affinity between GST-fusion protein and glutathione (GSH) using 'LbL' techniques.
The pairing of No Man's Land with Godot is a stroke of genius, bringing the two masters of comedic and enigmatic incomprehension, Pinter and Beckett, to the stage in alternating performances that underscore the affinity between them.
Overall, the transcription factor-binding affinities of the alternate alleles of rs10929728 and rs820336 34 were observed to be different.
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