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Therefore, to stabilize the complex we altered the sequences of the clamp binding motifs of PolIIIα and the exonuclease to increase the affinity for the clamp.
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As their experience increased, they rarely altered the sequence of flowers they visited.
A comparison of successional trends in adjacent uncut strips with the trends in the cut strips indicates that cutting has altered the sequence of successional changes in forest composition increasing the abundance of some species that were of low importance prior to cutting.
The early change in its expression may nevertheless have permanently altered the sequence of events leading to new neuron formation.
Despite the positive results promoted by the use of marijuana before crack, some of the interviewees altered the sequence of this combination due to the unfavorable environment created by this drug, which made the use of crack cocaine more difficult.
RT-PCR and relative gene expression experiments demonstrated that insertion of Himar1 into omp10 at nucleotide 245 from the start of the ORF altered the sequence of this gene.
In contrast to the repeat expansions located within coding regions, expansions located outside of coding sequences are usually very large and do not alter the sequences of the affected proteins.
As well as affecting transcriptional regulation, repeat sequences can alter the sequences of genes without disrupting their function.
Another strategy is to use mutagenesis to knock out the allergen genes, or possibly to alter the sequences of major allergenic epitopes in those proteins.
With more than 95% of genes undergoing alternative splicing [ 38, 39] in the human genome epigenetic histone deacetylation and other modifications such as methylation in cancer cells can alter splicing for the vast majority of genes and thereby alter the sequences of many RNA transcripts.
This chapter describes posttranscriptional modifications of the RNA in the EC, utilized to introduce changes in the RNA without altering the sequence of the DNA template and to address the effects of transcript composition on EC stability and catalytic activity.
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