The phrase "altered spot" is correct and usable in written English.
It can be used to refer to a location or area that has been changed or modified in some way.
Example: "The artist decided to paint over the altered spot on the canvas to restore its original beauty."
Alternatives: "modified location" or "changed area".
And, second, I wanted to see how one of the most massively altered spots on the planet held up ecologically when 6percentt of the U.S. population was crammed in there.
Quantitatively altered spots were excised from gels and subjected to mass spectrometry (MS) for protein identification.
Total protein amounts changed at each stage were calculated as sums of the spot volumes of all significantly altered spots.
Only fold changes over 10% were considered for graphs shown but results were similar when all significantly altered spots were included.
The clustered heat map for three experiments with 12 samples and 129 significantly altered spots is depicted in Figure 4B.
Proteomic 2D-DIGE analysis and mass spectrometric analysis of abundance altered spots resulted in identification of 69 protein spots with 23 being more intense in kairomone exposed animals and 46 less intense.
The most significant changes (1.5-fold, p < 0.05) between control and treatment groups were selected for protein identification by LC-Chip-ESI-QTOF-MS. Except for unidentified proteins due to weak spectra, 22 altered spots were identified (Table 2).
The traditional quantitation approach, which relies on high-resolution protein separation by 2-D gels and mass spectrometry identification of certain significantly altered spots, has been used for probing changes of O-GlcNAc proteins from several cell lines and tissues [ 75- 78].
In total, 41 significantly altered protein spots were detected, of which 34 spots were identified by MALDI-TOF/TOF analysis and found to represent 25 distinct gene products.
With the use of two-dimensional gel electrophoresis (2-DE), two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) and mass spectrometric (MS) technologies, 44 and 23 altered protein spots were identified in the cortex and hippocampus, respectively, in VAD mice.
Various methods have been employed to search for caspase substrates, including direct cDNA pool expression strategies or two-hybrid cloning approaches.5,6 By comparative two-dimensional (2D) gel electrophoresis of healthy and apoptotic cells, often a few hundred altered protein spots can be detected.
