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However, the preparation and storage of slices, as well as the flow rate of the solution during recordings, were altered: slices were kept in an interface-type chamber before recordings, and a higher flow rate was used in the recording chamber.
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Because the climbing fibre input is altered in slices, we verified the results obtained in cerebellar slices by chronically infusing the Fc chimeras for 6 days with osmotic minipumps inserted into the cerebellum.
PPF experiments confirmed that pre-synaptic function was not significantly altered in slices from FKBP12 cKO mice [29] or in slices from WT mice treated with Aβ1-42 (Figure 4D).
In our model, BLBP-positive radial glia, although substantially less disrupted, were not significantly altered when slices were exposed to reelin or NRG1.
The basic membrane properties of striatal MSNs have been shown to be progressively altered in slices from R6/2 mice (Cepeda et al., 2001, 2004; Klapstein et al., 2001).
Some of the effects of Aβ may therefore be lost or altered after slice preparation, as the extracellular Aβ concentration declines from the high picomolar to the low picomolar range.
The amplitude of mEPSC, which reflects the function of postsynaptic AMPA receptor, however, is not altered in neostriatal slices [20].
(While I was in Leipzig, I watched a graduate student cut the heads off some of the altered mice and then slice up their brains, like radishes).
So the slice backhand altered that tactical dynamic.
To assess the reproducibility of MR measurements, mice (n = 3) were reimaged under different conditions, including altered positioning in the coil, slice selections and imaging at different times.
You are correct, although acute slices may also have altered conditions.
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