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Remarkably, many identified rare variants include missense genetic alterations whose functional consequences are rather difficult to assess.
The identification of regions of breaks may provide a repertoire of alterations whose study may reveal interesting candidates.
Laboratory evolution is an important tool for developing robust yeast strains for bioethanol production since the biological basis behind combined tolerance requires complex alterations whose proper regulation is difficult to achieve by rational metabolic engineering.
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Integration of such functional RNAi data with gene expression and aCGH data has enabled us to identify genes that are targets of genetic alterations and whose expression is required for the maintenance of the malignant phenotype.
Taking Showa crater of Sakurajima volcano as an example, Miyagi et al. (2010) showed growth and disappearance of a shallow alteration zone whose growth was promoted by fluids associated with newly intruded magma.
The genes included in a panel of amplicon sequencing (actionable genes) are genes that are often altered in different cancer types, and for whose alterations targeted therapies have been established or are in clinical development.
Instead, we made specific alterations to the portion of the nBMP2 gene that encodes the bipartite NLS, whose alterations were predicted to block nuclear translocation of nBMP2 yet still allow synthesis and secretion of conventional BMP2.
Several important physiological functions require the cooperation of microbiota/microbiome, whose alterations play an important role in several human diseases.
This is in keeping with their roles in basic cellular functions (endo/exocytosis, vesicular trafficking, nucleocytoplasmic trafficking, cell signaling, proliferation and apoptosis, gene regulation, F-actin dynamics), whose alterations are associated with various types of diseases, in particular cancer, neurodegenerative, cardiovascular, and infectious diseases.
One potential biomarker to determine radioresistance is TP53 whose alterations are predictive of poor radiation response.
Repeats whose alterations are limited to substitutions of nucleotide bases (bp) are commonly called fuzzy tandem repeats.
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