Although other reports highlighted higher abundances of CBP/p300 and HDAC1 proteins in CRC tissue [ 34, 35], it must be stressed that the alterations in the expression of these epigenetic enzymes are not entirely responsible for specific histone modification levels, because other variables such as substrate availability and enzyme activity may contribute to the final modification.
The importance of peripheral muscle ischemia-reperfusion and rhabdomyolysis as a significant factor in the renal alterations was also stressed in other studies [ 4, 31].
However, apoptotic alterations were more pronounced in stressed animals, suggesting that stress related oxidative damage could have primed apoptotic effects of fluoxetine.
Were stress alterations significantly large enough?
As oxidative stress participates in skeletal muscle mitochondrial dysfunction, including altered biogenesis (27), it is tempting to speculate that fructose-induced mitochondrial alterations were related to oxidative stress.
Overall, carotid arterial stiffness was reduced (p < 0.01), but metabolic stress-specific alterations were not apparent.
Relevant stress-dependent alterations were found only between female samples, but not between castrated male samples.
In particular, relevant stress-dependent alterations were only found between female samples: Comparing HS-females and LS-females 1,038 probe-sets differed significantly (315 probe-sets: HS-females > LS-females).
Notably, the transcriptome alterations were highly related to hormone and stress responses, and many top up-regulated genes in 4× were associated with stress response.
Restraint stress-induced behavioral alterations were assessed using the hole-board, social interactions and open field tests.
The stress related behavioral alterations were assessed using the actophotometer, the hole board, the open field and the social interaction tests.
