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The phrase "alterations were observed on" is correct and usable in written English.
It can be used in contexts where changes or modifications have been noticed in a particular subject or situation.
Example: "After the experiment, alterations were observed on the test subjects' behavior, indicating a significant impact from the treatment."
Alternatives: "changes were noted in" or "modifications were detected in".
Exact(2)
In contrast, no significant alterations were observed on the endosteal surface at any loading frequency (p = 0.75 – 0.99; data not shown).
Multiple discrete alterations were observed on chromosome arms frequently implicated in breast cancer, including 1p (four deletions), 8q (eight amplicons) and 17q (seven amplicons and four deletions).
Similar(58)
Complex alterations involving multiple levels of change were observed on chromosome arms 1p, 8q, 9p, 11q, 15q, 17q and 20q.
No alteration was observed on the measures of left ventricular ejection fraction.
Incipient siliceous alteration was observed on basalt surfaces as young as 8 days old, but periods of a year or more are required to develop contiguous coatings with obvious opaque coloration.
In 73 rams, clinical alterations were observed by palpation, mainly on epididymes (n = 60; head and tail hypertrophy, indurations and nodules) and testicles (n = 13; asymmetry, indurations, degeneration and atrophy); 17 alterations were bilateral, 56 unilateral.
The same MR signal alterations were observed in lymph nodes that were not enlarged on CT.
Phenotypic alterations were observed only in flowers.
However, some remarkable alterations were observed.
After caffeine consumption no significant alterations were observed.
No significant alterations were observed in the MPH groups.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com