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Tissue alterations were evaluated semi-quantitatively by the degree of tissue change (DTC).
Clastogenic effects and histopathological alterations were evaluated after 4 and 8 months, respectively.
Plasmatic markers, and antioxidants defences systems were assessed and histological alterations were evaluated.
The biofilm formed on the blocks was analysed for F and Ca. Enamel alterations were evaluated by the percentage of surface microhardness change (%SMHC), cross-sectional microhardness (% mineral volume) and alkali-soluble F analysis.
Leucopoietic and erythropoietic alterations were evaluated by differential counting of 500 cells with reference to the cellular classifications of Penny [18] and Jain [19].
Genomic alterations were evaluated according to the Genome Alteration Print (GAP) methodology [ 20].
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The effects of the alterations are evaluated by testing well-known problems from previous studies.
The dynamics of cell morphology alterations was evaluated by confocal and time-lapse microscopy.
However, the prognostic value of individual segmental chromosomal alterations was evaluated in this analysis, rather than genomic types.
Another possibility is to use a systems biology approach, in which genetic alterations are evaluated in the context of pathways, networks and functional modules [ 5- 7].
Then, a subset of identified genetic alterations was evaluated in an independent sample set of 19 PDAC cases using fluorescence in situ hybridisation (FISH) analysis.
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