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For both the homozygous age3 deletion strains and the reconstituted strains the expected structural genomic alterations were confirmed by both PCR (not shown) and Southern blots (Fig. S1).
The mutations were introduced into the plasmid pRPCaMDR1-GFP according to the manufacturer's instructions, and the desired nucleotide sequence alterations were confirmed by DNA sequencing of the ORF.
PCAF sequence alterations were confirmed subsequently in genomic DNA.
These alterations were confirmed by western blot analyses.
Alterations were confirmed by sequencing cDNA derived from the LCLs of patient A-T213.
All sequence alterations were confirmed after a repeated extraction of DNA.
Similar(46)
The importance of these structure alterations was confirmed by the twofold growth of the quantum yield of anthracene photo-oxidation catalyzed by tetraphenylporphyrin (TPP) immobilized on MF-4SK membranes treated with ethylene glycol.
Nevertheless, a surface-chemistry alteration was confirmed by X-ray Photoelectron Spectroscopy.
Bacterial activity and biodegradation were determined by adenosine triphosphate content and 1H NMR spectroscopy, respectively; surface alteration was confirmed by SEM analysis.
Wettability alteration was confirmed by contact angel measurements demonstrating rock wettability shift from slightly oil wet to medium water wet condition as we increase the IL concentration in the aqueous phase.
Each alteration was confirmed at least twice by PCR.
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