In order to investigate the molecular basis of the FRG1 transcriptional alteration, we made a detailed analysis of the chromatin structure of two DNA regions residing in the FSHD locus in a human model of myogenic differentiation: the candidate gene FRG1 and the D4Z4 array, to which the genetic mutation underlying the disease has been mapped.
Instead, we made specific alterations to the portion of the nBMP2 gene that encodes the bipartite NLS, whose alterations were predicted to block nuclear translocation of nBMP2 yet still allow synthesis and secretion of conventional BMP2.
Once this was agreed upon, we made any alterations that were required and laser scanned the model.
"We made some alterations around the team to try and get back in the game and credit to my players for getting back into the game.
We made similar alterations later in the Discussion and in the legend for Figure 10.
In order to secure the confidentiality of the participants, we made minor alterations to prevent identification of cases (e.g., names and places that could identify the participant).
We made an alteration in this method of classification, adding one new class, the In-Cab Train Advisory Systems, reorganized the content of some classes, and extended the description of each class and their subsystems.
POS supports nearly all the Pig operators and built-in functions, except that we make some alterations on operators and functions to adapt to real-time features.
But if we make similar alterations to Billy's throw while holding Suzy's throw fixed, we find that the shattering is unchanged.
Sometimes, we make the alterations intentionally -- our friends suggest, our partner asks, our boss demands we change our ways -- and sometimes we make the alterations unconsciously.
