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Graphical representation of genomic alterations was performed with VAMP software (Institut Curie, Paris, France) [ 15, 16].
Screening for dosage alterations was performed by oligo-CGH-array with no relevant findings (GSM1527006).
Correlation of gene expression data with genomic alterations was performed as described ([ 27] and Additional file 2).
Analysis of transcript expression alterations was performed using the Affymetrix U133 Plus2.0 array on 16 IDC samples.
The classification of gene alterations was performed in accordance with the entries in the Breast Cancer Information Core BICC, Bethesda, MD).
An evaluation of all classes of genomic alterations was performed, including base substitutions, short insertions/deletions, focal amplifications, homozygous deletions, and gene fusions/rearrangements, as previously described 3.
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Analysis and quantification of the histopathological alterations were performed by 2 independent investigators, including an unrelated expert pathologist who was blinded from the experimental design and the treatments the animals received.
The method of CGH-analysis, the corresponding control experiments and the criteria for the evaluation of genetic alterations were performed as previously described (Kallioniemi et al, 1994; Buerger et al, 1999b).
To this aim, the definition of the dose range for different tracers and the quantitative assessment of detection borders of anatomical and molecular alterations were performed in physiological and pathophysiological mouse models by means of the MPS.
Thirteen outliers were found in sleep diary data, and following the recommendations by Tabachnick & Fidell [ 45], score alteration was performed in order not to lose valuable data, but lessen the impact of outliers.
Rule 2 The sequence alteration is performed for each block of "NLV" vehicles.
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