The phrase "alterations was determined" is not correct in written English.
It should be "alterations were determined" to agree in number with the plural noun "alterations."
Example: "The alterations were determined after a thorough review of the project requirements."
Alternatives: "changes were established" or "modifications were decided."
Overall, 768 somatic alterations were selected for further curation after initial prioritization by PHIAL 15 67% (511/768) of these somatic alterations were determined to be potentially clinically relevant and reported to the clinical team (Figure 1a, Supplementary Figures S2 S4 online, Supplementary Table S5 online).
CSE induced ultrastructural alterations were determined by electron microscopy, confirmed by Western blot and physiological consequences studied in open-flow biofilms.
These alterations were determined using genome BAC array CGH [26], [27], [28], [29] comprised of 2464 BACs selected at approximately mega base intervals along the genome as described previously [26], [28].
Morphological alterations were determined by light microscopy, transmission and scanning electron microscopy.
In addition, potential organ-specific alterations were determined within the total group of hrHPV-positive SCCs.
The genomic coordinates for these alterations were determined through a local mapping using BLAT and crossed against surfaceome genes coordinates.
Occult metabolic alterations were determined by calculating the lactate- pyruvate (L/P), lactate- glucose (L/Glc), and lactate- glutamate (L/Glu) ratios.
In order to evaluate the clinical relevance of SMAD4 deletion, gene copy alterations were determined by copy dosage using real-time quantitative PCR in 202 colorectal tumour biopsies from a previous randomised study of adjuvant chemotherapy.
The segmentation constant, c, was set to 8. Copy number alterations were determined by comparing the segmented log2 ratios to gain/loss thresholds obtained by an adaptive scaling method [ 22], using a window size of 2% and a scaling factor of 2. Segments were accordingly designated gained, lost or not changed, giving a ternary scale (-1, 0, 1).
Mutation status in the samples were analyzed by algorithms developed by Personal Genome Diagnostics Inc. (Baltimore, MD, U.S.A .. WES data were mapped to the reference human genome sequence and sequence alterations were determined by comparison of over 50 million bases of tumor and normal DNA.
