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Our data indicate no loss of adherence of DFMO-treated bacteria, suggesting that other alterations of bacterial physiology are responsible for the reduction in IL-8 expression.
Following internalization, extensive alterations of bacterial gene expression were observed.
Similarly, alterations of bacterial folate metabolism modulate C. elegans lifespan (Virk et al, 2012).
Detailed post-interventional alterations of bacterial groups on all taxonomic levels were identified using coefficient plots of OPLS-DA models (SI-Figure 2 and SI-Table 1).
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In this study we investigated the relationship between a rapid change in extracellular pH and the alteration of bacterial ATP concentration.
The antimicrobial and tissue-digesting capabilities of sodium hypochlorite are owed to chemical reactions that result in an alteration of bacterial cell metabolism as well as lipid and fatty acid degradation of cell membranes.
Our current findings suggest that this effect could involve direct alteration of bacterial metabolism as well as the known inhibition of host cell ODC.
TEM monographs showed that vancomycin and the extract caused rupture of bacterial cell wall and alteration of bacterial shape compared to control.
Moreover, as evident by TEM monographs, the extract caused rupture of bacterial cell wall and alteration of bacterial shape compared to control.
We conclude from this analysis that exposure of bacteria to our experimental parameters prior to cellular internalization does not result in detectable alteration of bacterial transcriptome.
In order to quantify the alteration of bacterial wall, the thickness of bacterial cell wall was measured by using image analysis software (Image-Pro Plus 6.0, Media Cybernetics Inc., Silver Spring, MA, USA).
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