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Coordinate alterations were validated with RNA-seq data from black (n = 49) and white patients (n = 216).
Sequence alterations were validated with a second independent PCR.
The array CGH analysis for copy number alterations was validated by FISH.
KCNQ1OT1 and H19 alterations were validated by methylation specific multiplex ligation-dependent probe amplification (MS-MLPA) using SALSA ME030; and SNRPN alterations were validated with SALSA ME028 (MRC-Holland, Amsterdam, Netherlands).
The identified miRNA expression alterations were validated by real-time PCR, and tissue distribution of the miRNAs was visualized by in situ hybridization.
This predicted pathway alteration was validated with an animal model of heart failure (HF).
Candidate SNPs including those which might interfere with miRNA biogenesis or target alteration were validated by PCR analysis using the DNA from 20 wild common carp.
Degrees are validated by University of Wales Trinity Saint David.
Sometimes assumptions are validated.
You are validated.
They are validated.
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