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Identification of somatic alterations was performed as previously described32.
Graphical representation of genomic alterations was performed with VAMP software (Institut Curie, Paris, France) [ 15, 16].
Analysis of transcript expression alterations was performed using the Affymetrix U133 Plus2.0 array on 16 IDC samples.
Screening for dosage alterations was performed by oligo-CGH-array with no relevant findings (GSM1527006).
The classification of gene alterations was performed in accordance with the entries in the Breast Cancer Information Core BICC, Bethesda, MD).
Correlation of gene expression data with genomic alterations was performed as described ([ 27] and Additional file 2).
The comparison of gene expression levels in samples with and without copy number alterations was performed as was described before for the qRT-PCR analysis.
Evaluation of genomic copy number alterations was performed using Affymetrix Copy Number Analysis Tool software (CNAT, version 4) where the threshold for statistical significance was P≤10−6 as recommended by Affymetrix.
An evaluation of all classes of genomic alterations was performed, including base substitutions, short insertions/deletions, focal amplifications, homozygous deletions, and gene fusions/rearrangements, as previously described 3.
Testing for large genomic alterations was performed by MLPA using the SALSA MLPA Kit P002B BRCA1 (MRC-Holland) as described by Schouten et al. [ 25].
Because outcome measure of behaviour alterations was performed in the first few days after birth, any positive finding may be subtle and not relevant in the long-term outcome.
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