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Alteration frequencies and frequency differences in MSS samples are shown in Figure 1.
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GISTIC differs from these methods in that it assesses the statistical significance of the combination of alteration frequency and magnitude across the genome.
Data on DDHD2 copy number alteration frequency and patient survival in patients with different cancer types was generated using cBio portal (http://www.cbioportal.org/public-portal/) [ 50].
Correlations were tested using the Pearson correlation analysis based on the data for alteration frequencies calculated based on the AFLP and MSAP variant frequencies and altered gene expression.
The frequency of SCNA across the whole genome was assessed to compare alteration frequencies between primary tumors and metastases.
Although several genes involved in Wilms' tumour have been identified, as described above, the alteration frequencies of these genes (loci) and how many loci are altered in the tumour are still unknown.
Modules were visualized as discrete colors and gene alteration frequencies were visually resized in proportion to alteration frequency across the 84 analyzed GBM cases.
Finally, regions which passed the first two criteria and demonstrated alteration frequencies differing by at least 20% occurrence in either copy number loss or gain were selected for further analysis.
Alteration frequencies were compared using the Fisher's exact test and false discovery rate (FDR) was applied to correct the multiple testing hypothesis (p<0.05) [51].
However, by ranking the pathways according to their variability in alteration frequencies between cancers, we identified several pathways that were altered very frequently within one or few cancer types and only rarely in other cancers.
Parent-of-origin has a conspicuous effect on the alteration frequencies.
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