This is likely due to the original "knock-in" strategy not deleting ACTN coding sequences, but rather the construct appeared to alter transcription or transcript stability.
The most direct way to perturb a transcriptional network is to alter transcription factor activity.
Although the precise mechanisms involved in this enhanced apparently stable phage resistance were not clear, these data suggest that autoinducers might promote some sort of epigenic change (e.g., a change in DNA methylation that might alter transcription) or a specific genetic alteration that could drive changes in O1 antigen expression.
This suggests that the SNP variant does not alter the protein product significantly, but may alter transcription factor binding leading to altered expression patterns [ 14].
Although ubiquitylated H2B does not alter transcription through chromatin on its own, the increased stimulation of transcriptional elongation in the presence of FACT suggests that the ubiquitin modification either intrinsically destabilizes the nucleosome or assists destabilization through action of FACT.
In our settings, resveratrol did not alter transcription of genes previously described as responding to CR or the stilbene itself.
Arbibe, L. et al. An injected bacterial effector targets chromatin access for transcription factor NF-κB to alter transcription of host genes involved in immune responses.
It is well established that variants in HERC2 alter transcription of the neighbouring pigmentation gene OCA2 which is additionally associated with blue eyes and blonde hair colour12,21,22,23,24. Recessive mutations in OCA2 result in albinism.
AS-ODNs can alter transcription (by preventing binding of transcription factors), RNA processing (by binding to sequences necessary for RNA splicing), or translation (by binding to the translational initiation codon).
The success of chronic GC therapy for asthma stems largely from the ability of the GC GC receptor (GR) complex to alter transcription of a wide array of molecules involved in the inflammatory process.
Thus, GLUT2-mediated glucose sensing did not significantly alter transcription and stability of insulin mRNA.
