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Most importantly, microCT does not damage or alter the specimen in the least, and sections of the cones in the three planes (transverse and the two longitudinal planes) are obtained without specimen loss due to sawing, grinding, or reorientation of the specimen for cutting in another plane of section, as is the case with traditional thin-sectioning.
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On the other hand, similar procedures were followed in conducting the thermal fatigue test by altering the specimen's temperature between (16 ± 2 °C) and (40 ± 2 °C).
In fact, the dislocations distributed in the specimen alter the machining-induced surface mechanical properties.
Related changes in structure and the effect of capillary pressure can alter the properties of the specimen and affect the reliability of the test results.
Another possibility might be the usage of coatings with thicknesses of tens of nm, that on the one hand would lead to higher protection factors, but on the other hand would alter the properties of the specimen and reduce the TEM image contrast.
The use of 5 μl of unextracted clinical specimen did not appear to significantly alter the crossing points of either specimen relative to extracted sample.
Splicing alterations in CLL specimens can alter the type of transcripts and thereby function of a large number of cellular proteins that may provide the cell with survival advantage.
Any upgrading of the Gleason sum from the biopsy to the final pathologic specimen may alter the treatment options.
Thousands of uncontrolled variables that affect every tissue specimen could alter the results of companion diagnostic testing.
Also the altered shape of the rudiments in immobilized specimens may alter the forces but this is unlikely to have a large effect since we previously found that the general pattern of the forces do not change dramatically with shape changes over time [30].
Since delayed time to fixation can alter the phosphorylation status in resection specimens, use of these epitopes in companion diagnostic tests will likely have to be limited to core needle biopsies.
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