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Antigen retrieval was performed using the ALTER technique as previously described (Reynolds et al, 2002) in ethylene diamine tetraacetic acid (EDTA) buffer with Tween 20 overnight at 65°C.
Antigen retrieval was performed using the ALTER technique (Reynolds et al, 2002) and staining with the monoclonal antibodies to BMI-1 (229F6; Upstate, Watford, UK; 1 : 100) and 6C9 (1 : 10) was detected using Vector NovaRed (Vector Laboratories, Peterborough, UK) or DAB (Dako, Ely, Cambs, UK).
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Weight was lost, diet altered, technique improved, mindset hardened.
Later he quotes American anthropologist Donald Brown's celebrated work Human Universals, which lists "mood- or consciousness-altering techniques and/or substances" as one of the essential components of human culture, along with "music, conflict resolution, language and play".
Students executed the sub-tasks three-dimensionally by using altered techniques and materials; drawing was not allowed.
The Truth in Advertising Act asks the Federal Trade Commission (FTC) to develop what's called a "regulatory framework" for ads that significantly change the people in them through image-altering techniques like "Photoshop".
Furthermore, altered techniques used for the detection of microalbuminuria resulting in a reported prevalence of microalbuminuria ranging from 10%to40%0% [ 29, 30].
Unable to alter a technique they clearly considered out of date, he anticipated further savaging at their hands for any subsequent volumes.
An Australian player wishing to alter his technique must commit time and — if he wants to learn from Chapman — significant money to make it happen.
I learn everything once and I never alter my technique, even when I find out about better ways to do things.
If you are not happy try again but alter your technique until you get the results you want.
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