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However, biochemical analysis will not identify some quantitative defects of type I procollagen, certain causative variants that alter sequences in some coding regions of the COL1A1/COL1A2 genes and recessive forms of OI (including Bruck syndrome) that result from variants in FKBP10, PLOD2, SERPINF1, SERPINH1 or SP7.
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The formalin introduces crosslinks that can both fragment DNA and cause chemical alterations that may alter sequencing results [ 19].
In addition, the type of promoters has been reported to alter sequence diversity at the promoter region.
A failure to identify all isomiRs can alter sequence counts and lead to incorrect conclusions of miRNA abundance [ 13].
54 Post-transcriptional changes in the V3 or V4 regions of gp120 that alter sequence or glycosylation may determine the course of disease as they have been correlated with the incidence of HIV-associated dementia.
However, the rare clasts of chalcedonic quartz are the erosional products of the hydrothermally altered sequences of the Valhazir formation (SKM 2005a, b).
Plasmids containing the original and the altered sequences were electroporated into B. neotomae and the level of β-galactosidase expression was determined using 3 separate colonies for each plasmid construct.
Some previously characterized lysozyme g sequences are suggested to have altered secretion potential and enzymatic activity, however the distribution of these altered sequences is unknown.
All the 33 sequenced clones had altered sequences, including 6 types of mutations in embryo #1-1 (17 of 17 sequenced clones; 100%) (Fig. 1D) and 7 types of mutations in embryo #1-2 (16 of 16; 100%) (Fig. 1E).
The overwhelming majority of the altered sequences are single domain Hrs, implying that their function may be less important to the survival of the organism than the chimeric Hrs.
We also generated mutant reporters (Δs1, Δs2, and Δs3) that have altered sequences in the Nanog-binding sites, which prevented Nanog-induced luciferase activity, suggesting that the presence of these NREs is required to activate transcription of miR-17-92 miR-17-92 miR-17-92
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