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Syndecan-1, also named CD138, is expressed by normal epithelial cells but is also transiently expressed in condensing mesenchyma during embryonic morphogenesis [4].
NOTCH inhibition also transiently delayed leukemia progression in vivo.
Suppression of TLX1 in vivo also transiently delayed leukemia progression.
As previously mentioned, activated T cells can also transiently upregulate Foxp3 expression levels [27].
Eag1 did not only slow-down, but got also transiently stabilized inside synapses.
To determine if the PPXY motifs of LITAF affected Nedd4 localization, YFP-Nedd4 was also transiently co-transfected with the LITAF double mutant (myc-LITAF Y23A,61A).
The cDNA construct was stably expressed in Chinese Hamster Ovary (CHO) cells (CHO-GPI-TSHR-ecd) and also transiently expressed in Human Embryonic Kidney (HEK) 293 cells.
Interestingly, the application of diazoxide (DZ) was unable to replicate the action of glucose free even though DZ also transiently reduced [Ca2+]i (Figure 4C).
Foxp3, a transcription factor considered by many as the Treg marker, appears to be also transiently expressed in activated Tconv [15].
Both WT ACTN4 and ACTN4 KD fibroblasts were transiently transfected with eGFP and ACTN4 KD fibroblasts were also transiently transfected with human wild type ACTN4 tagged with eGFP as above.
In parallel we also transiently expressed an N-terminal construct of ATL9, 35S GFP ATL9, in tobacco with a 35S GFP construct as an internal negative control for GFP localization to the nucleus.
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