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Any of the reaction steps taking place in situ may have also required a higher mole fraction of the CDNBD reagent, leading subsequently to the 2 1 reagent to drug ratio.
In addition to tossing religious freedom into the mix, Virginia's case also required a higher burden of proof, which gave the government its first chance to defend the executive order.
The induction of growth also required a higher demand for the methylation of tRNAs and rRNAs.
The cells from the adult brain also required a higher plating density to produce spheroids (3×10 cells/ml).
The latter approach resulted in a higher volumetric activity but also required a higher substrate loading (' Production of (hemi-) cellulases by T. reesei SVG17').
Consistent with this observation, inhibition of autophagy in MEFs also required a higher concentration than in other cell lines (Supplementary Figure 3).
Adoption also requires a high overhead.
Pervasive uncertainty also requires a high degree of flexibility and a capacity for program revision.
That would make them core, but it also requires a high level of trust.
They also require a high level of collaboration and consensus-building, he said, under sometimes tense political conditions.
It also requires a high degree of practical knowledge, a capacity for undertaking the political actions sufficient for bringing about the actions required and a citizen body willing to adopt and accept the proposed political system.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com