In addition to the five genes successfully cloned in this study, another two genes available in Genbank, BC and SAD, were also employed for gene expression test.
The Fisher's exact test was also employed for pairwise comparison of distributions of categorized groups.
Since this was a separate analysis the t test was also employed for this question, recognising the limitations of using any statistical analysis with just six cases.
The ABI 7500 fast SDS software was used to determine the relative copy number of T. This approach was also employed to test other genes for residency within the deleted region.
It should be noted that betweenness and closeness centrality, which are often also employed to test network vulnerability, are too computationally inefficient to be considered for these networks.
Although this study relies on parametric methods to generate conclusions, non-parametric Wilcoxon rank sum testing was also employed to provide sensitivity testing for robustness and reliability of results.
We also employed statistical tests for small study effects.
This software was also employed to obtain insights for testing likely deviations from neutrality, through the computation of Tajima's D [ 78] and Fu and Li's D* [ 79] tests.
Regression and correlations analyses were also employed as tools for statistical tests and to establish trends and relationships between crown cover and carbon stocks, and shade tree diameter and carbon stocks and Shannon-Weiner Diversity Indices H' H max and J.
In the context of this test, we defined "enrichment ratio" as: 4 We also employed this test to assess, separately for each study, whether significantly altered genes were biased for specific chromosomes (Additional file 12: Table S6).
Epi Info version 6 was also employed in conducting Mantel-Haenszel test to adjust for possible confounding effect of age on the variables.
