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We also confirmed the interaction using the ITC measurement (Fig. 2C).
From the spectra, the intense broad band (Figure 1b d) observed at 3500 cm−1 was assigned to the OH stretching vibration which was mainly observed when steric hindrance prevents polymeric association and also confirmed the interaction of nHA with PPEES.
Bimolecular fluorescence complementation (BiFC) assays also confirmed the interaction between SPL and TPL in vivo.
They also confirmed the interaction of importin- α3 with TG2 in non-small cell lung cancer cell lines by immunoprecipitation.
We demonstrated the FN-binding capability of EF-Tu using a ligand blot assay and also confirmed the interaction with FN in a dose-dependent manner by ELISA.
Similarly, co-immunoprecipitation analysis with integrin β1 antibody using PST080 ES cells also confirmed the interaction of endogenous integrin β1 with ΔCD98hc-β geo fusion protein.
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This experiment also confirms the interaction between Nox4 and the subunit p22phox.
In vivo experiments and in vitro yeast two hybrid assays also confirm the interaction between TNFAIP3 (zinc finger protein A20) and TAX1BP1.
The proteomic analysis of the p160-containing protein complex also confirmed the physical interaction of p160 and the VHL ubiquitin ligase complex (Figure 3): FLAG-p160 was transfected into 293T cells and the protein complex containing FLAG-p160 was purified by anti-FLAG immunoprecipitation under non-denaturing conditions.
We also confirmed the common interaction of LNX1, LNX2 and MUPP1 with EphA7 and show that EphA7 can bind, not only to an isolated LNX1 PDZ domain [ 42], but also to full-length LNX2 and near full-length LNX1 proteins.
The multiple regression analysis also confirmed the decreased interaction of PMd and M1 within the dorsomedial circuit (PMd M1: t(14) = 2.84, p = 0.013; Figure 3 figure supplement 1B,D,E); the response of PMd to activity in pSPL showed a tendency to be decreased (PMd-pSPL: t(14) = 1.78, p = 0.097; Figure 3 figure supplement 1C,D,E).
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