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The reach was also classified using the Rosgen Level 2 stream morphology, classification system (Rosgen 1996).
Patients with metastatic disease were also classified using the International Germ Cell Cancer Collaboration Group (IGCCCG) prognostic scoring scheme, which divides metastatic GCT into good, intermediate and poor prognosis groups (IGCCCG, 1997).
However, as an ORF can be poorly expressed under one condition but highly expressed under another, the ORFs were also classified using the same RPKM value ranges but only considering the Highest RPKM Value observed among the four conditions (HV grouping).
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The predicted proteins were also classified using LCA (at the same values for p), SOrt-ITEMS, CARMA3 and best BLAST matching at the same levels of novelty.
The mode of tumour invasion was also classified using a modification of the criteria of Jacobsson et al (Jacobsson et al, 1973; Yamamoto et al, 1983).
The sequences were also classified using network analysis according to whether they fall into groups that tend not to share polymorphic regions [ 33].
All patients were also classified using socio-demographic variables (age, gender, marital and family status), collected by GPs using the detection module.
While we used the 3F PTA values to classify hearing impairment in participants to comply with previous studies, 1 3 hearing impairment is also classified using a four-frequency average (4F PTA) of 0.5, 1, 2 and 4 kHz.
For verification of the results, remaining jobs as well as two other jobs were also classified using soft independent modeling algorithm (SIMCA).
Genes with known function were also classified using Gene Ontology database.
As reference parental species, we used 40 well-defined genotypes of D. cucullata, D. galeata and D. longispina, originating from 23 locations in Europe and one location in North America, which were also classified using two allozyme loci, recognised to be diagnostic for species identification [ 33, 43].
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