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The non-native producers, Escherichia coli and Saccharomyces cerevisiae, have also been engineered for 1, 3-PD production.
To allow access to alternative carbon sources, C. glutamicum has also been engineered for utilization of glycerol, pentoses, and amino sugars as well as polysaccharides (Schneider et al. [2011]; Rittmann et al. [2008]; Seibold et al. [2006]; Uhde et al. [2013]; Gopinath et al. [2011]; Matano et al. [2014]).
Z. mobilis has also been engineered for the production of sorbitol, gluconic acid, levan, 2,3-butanediol, isobutanol, and other biochemicals, which is proposed as an ideal microbial chassis for future synthetic biology and biorefinery (He et al. 2014; Yang et al. 2016).
Communication between cells has also been engineered for multiple applications, including pattern formation [11] and oscillators [12].
Other strains, including E. coli[ 132, 133], and S. cerevisiae[ 134, 135] have also been engineered for succinic acid production.
A. baylyi ADP1 has also been engineered for the production of valuable biochemicals, like cyanophycin [ 23], wax esters [ 24], and triacylglycerols [ 25].
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SiO2 nanoparticles (NPs), in addition to their widespread utilization in consumer goods, are also being engineered for clinical use.
Moreover, the bioactives can also be engineered for targeted delivery (eg, colon, CNS specific) with the desired/modified therapeutic effects.
To avoid recombinase-mediated off-effects, the recombinase could be expressed transiently, inducibly, developmentally, and it could also be engineered for auto-excision.
In addition, using enzymes mined from the biosynthetic pathways, E. coli has also been engineered to be a platform for biocatalytic synthesis of polyketide-based, clinically relevant drugs (Xie et al. 2007).
Naturally occurring enzymes have also been engineered to alter their activities and specificities for use in synthetic, environmental and medical applications [7].
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